FCS曲线
荧光自相关曲线
Fluorescence autocorrelation curve	
随着特征时间Tau的增加，自相关幅度G(Tau)逐渐衰减直至平衡
As the characteristic time Tau increases, the autocorrelation amplitude G(Tau) gradually decays until it reaches equilibrium equilibrium.
FCS曲线有明显衰减趋势，表明测试样品 “有信号”；若无，则表明样品“无信号”，原因为样品浓度太高、荧光团的光量子效率太低、样品几乎不扩散等
If the fluorescence autocorrelation curve shows a clear decay trend, it indicates that the sample “has a signal”; if not, it indicates that the sample “has no signal” because of exceeding sample concentration, low fluorophore quantum efficiency, or nearly non-diffused sample, etc.

Tau (μs-s)	
特征时间
Characteristic time	
自相关曲线的横轴
The horizontal axis of the autocorrelation curve	
1~10μs反映荧光团的光物理特性；10μs以后反映的是样品扩散的信息
1~10μs reflects the photophysical properties of the fluorophore; time after 10μs reflects the sample diffusion.

G(Tau)	
荧光自相关幅度
Fluorescence autocorrelation amplitude	
自相关曲线的纵轴
The vertical axis of the autocorrelation curve	
G(0)值为扩散起点(Tau = 10 μs)处的纵轴数值，G(0)值越大表明荧光团浓度越低，反之亦然
G(0) is the vertical axis value at the starting point of diffusion (Tau = 10 μs). The larger the value of G(0), the lower the concentration of fluorophore, and vice versa.

TauD
(ms)	特征扩散相关时间
Characteristic diffusion correlation time	
自相关幅度衰减到G(0)值一半时对应横轴的时间
The time on the horizontal axis when the autocorrelation amplitude decays to half of G(0).	
与样品的大小/体积成正相关，即样品越大，TauD值越大；与扩散系数D成反比
It is positively correlated with the size/volume of the sample; the larger the sample, the larger the TauD value. It is inversely proportional to the diffusion coefficient D.

N	
平均荧光分子个数
Average number of fluorescent molecules	
表示在任何时刻里，共聚焦体积内所检测到的平均荧光分子个数
Represents the average number of fluorescent molecules in the confocal volume at any time.	
与样品摩尔浓度成正比，浓度越高，N值越大
It is proportional to the molar concentration of the sample; higher concentration results in a larger N value.

Lambda (cps/mole.)	
平均荧光分子亮度
Average fluorescence molecule brightness	
代表每个荧光分子的发光亮度，与荧光团的发光性能(光量子效率)相关
Represents the fluorescence intensity of each fluorescent molecule, which is related to the luminescent properties (photo-quantum efficiency) of the fluorophore.	
特殊处理样品时，可用于判断样品的聚集状态。如单体分子亮度为1，则二聚体的亮度约等于2
When the sample is specially processed, it can be used to estimate the aggregation state of the sample. For example, if the lambda of a monomer is 1, then the lambda of a dimer is approximately 2.

Rhd (nm)	
流体力学半径
Hydrodynamic Radius	
与样品的大小呈正相关，与TauD值成正比，与D值成反比
Positively correlated with the size of the sample; proportional to TauD; inversely proportional to D.	
直接获得样品的尺寸，但细胞样品或液滴样品需要进行黏度校正
It directly provides the sample size, but cell samples or droplet samples require viscosity correction.

D (μm/s2)	
扩散系数
Diffusion Coefficient	
样品扩散的快慢
The speed of sample diffusion	
同Rhd 值
Same as Rhd

C (nM)	
摩尔浓度
Molar Concentration	
与G(0)值成反比
It is inversely proportional to G(0)	
通过检测通过共聚焦体积内的荧光分子个数来计算得到的摩尔浓度，不需要建立标曲即可得到
The molar concentration is calculated by detecting the number of fluorescent molecules within the confocal volume, without the need for a standard curve.

Intensity (cps)	
荧光总强度
Total Fluorescence Intensity	
与样品浓度、发光亮度相关，其值为分子亮度(Lambda)与分子个数(N)的乘积
It is related to sample concentration and fluorescence intensity. Its value is equal to the product of molecular brightness (Lambda) and the number of molecules (N).	
代表测试体系内所有荧光信号的强度，类似于荧光酶标仪所测得的荧光值
It represents the intensity of all fluorescence signals within the test system, similar to the fluorescence values measured by a fluorescence microplate reader.

Single 3D Diffusion	
三维单组分扩散
Single component 3D Diffusion	
单组分样品自由扩散
Free diffusion of a single-component sample	
水相纯溶液样品皆可使用此模型拟合数据，一般在10μs处开始拟合FCS曲线
This model can be used to fit the data of pure aqueous solution samples, and the FCS curve is generally fitted starting from 10 μs.

Double 3D Diffusion	
三维双组分扩散
Double components 3D Diffusion	
双组分样品自由扩散
Free diffusion of a double-component sample	
样品作自由扩散且体系内存在双组分时（尺寸差距明显）可使用该模型拟合，也为10μs处开始拟合
This model can be used to fit the data of samples with two components that exhibit free diffusion and have significant size differences. The fitting of the FCS curve starts from 10 μs.

Anomalous Diffusion	
异常扩散模型
Anomalous Diffusion Model	
样品运动并非自由扩散，受到一些其它因素影响的扩散
The movement of the sample is non-free diffusion influenced by other factors.	
常用于活细胞体系的数据拟合
It is often used for fitting data from living cell systems.

Single 2D Diffusion	
二维单组分扩散
Single component 2D Diffusion	
单组分在二维平面上的扩散方式
Diffusion pattern of a single component on a 2D plane. 	
常用于细胞膜上单组分样品扩散的拟合
It is often used for fitting the diffusion of single-component samples on the cell membrane.

Double 2D Diffusion	
二维双组分扩散
Double components 2D Diffusion	
双组分在二维空间上的扩散方式
Diffusion pattern of a double-component sample on a 2D plane.	
常用于细胞膜上双组分样品扩散的拟合
It is often used for fitting the diffusion of double-component samples on the cell membrane.

Triplet Dynamics	
三线态模型
Triplet Dynamics
Model	与荧光团的性质有关，模型拟合可根据FCS曲线选择该项拟合参数
It is related to the properties of fluorophores, and this fitting parameter can be selected based on the FCS curve.
1~10μs尺度下的FCS曲线若有明显下降，表明其三线态占比较高；若为平缓趋势时，表明占比低
If the FCS curve drops significantly between 1μs and 10 μs, it indicates that the proportion of the triplet state is high; if the curve is flat, the proportion is low.
